Recent advances in techniques for isolation and culture of primary hepatocytes
نویسندگان
چکیده
منابع مشابه
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15 صفحه اولcomparison of the amount of debris extruded apically in two rotary techniques: flexmaster and m2
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15 صفحه اولProgress in human hepatocytes: isolation, culture & cryopreservation.
The quality of the hepatocytes used for clinical cell transplantation is very important, and depends to a large extent on the nature of the tissue used for isolation. The collagenase perfusion technique to isolate hepatocytes from animal livers has been further developed for isolation of human hepatocytes. As the donor organ pool is a scarce resource, marginal livers unsuitable for transplantat...
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The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and purity are much lower in this condition....
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High levels of regulated oncogen-alpha (GRO-a) expression have been observed in the liver. GRO-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. Given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. In this study, hepatocytes (2×106 cell/ml) were...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2009
ISSN: 2219-2859
DOI: 10.11569/wcjd.v17.i21.2164